Cigarette butts from 5 smokers were gathered and then, placed in room temperature for 1, 3, 5, 7, 15 days. The possible use of the cigarette butts for individual indentification was evaluated in sex determination, amplification of D1S80 locus, polymorphisms of HLA-DQA1 gene from the extracted DNA.
1. DNA extraction was possible in cigarette butts were left in room temperature for 15 days, so it can be
applicatable to individual identification by polymerase chain reaction MR).
2. Amplification of X-Y homologous amelogenin gene by PCR made it possible to identify the sex in saliva
stains .(cigarette butts). .
3.´ Amplification of D1S80 locus can be ´ acquired from adding the bovine serum albumin and hot" start PCR ´ procedures firm forensic samples such as as saliva stains (cigarette butts), so the AMP-FLPs examining is possible.
4. Genotype could be detemuned simply and rapidly using Amplitypem HLA-DQa forensic kit in examining the HLA-DQA1 gene.
From the investigation, DNA extraction, sex determination, amplification of D1S80 locus, polymorphisms of HLA-DQA1 gene was successfully done even though the cigarette butts were left for 15 days at room temperature. Therefore cigarette butts are highly reliable and applicatable as molecular biologic samples for individual identification.
|